Human Consumption Research Articles

Genomic perspective of Salmonella enterica isolated from farm animals in Sinaloa, Mexico

Farm animals play a crucial role as a primary source of sustenance for human consumption. However continues to be one of the major food-borne pathogens from a public health standpoint. Its persistent global concern surrounding stems from its capability to induce foodborne illnesses. Unraveling the genetic characteristics of this pathogen stands as a pivotal step, shedding light on the intricate biology of and aiming to mitigate its prevalence. Within this context, our study aims to delve into the genomic traits and population structure of Mexican isolates derived from farm animals. We performed next-generation sequencing across 72 genomes. Genomic data was analyzed to determine virulence and antibiotic resistance markers. In addition, a phylogenetic tree and a spanning tree was constructed. The study revealed a diversity of 18 serovars linked to a singular ST, with prominent serovars being Oranienburg, Give, and Saintpaul. Across all isolates, an extensive array of virulence-related genes was identified. Interestingly, 95% of the isolates displayed exclusive resistance to aminoglycosides, while the remaining exhibited multidrug resistance to tetracycline and chloramphenicol. Notably, a substantial prevalence of prophages in the genomes was observed, accounting for 94% and totaling 183 sequences. The serovar Give stood out with the highest number of sequences, featuring Vibrio X29 and Escher RCS47 as the most prevalent phages. This comprehensive analysis provides valuable insights into the intricate world of , paving the way for enhanced understanding and targeted interventions.

AFB1 Toxicity in Human Food and Animal Feed Consumption: A Review of Experimental Treatments and Preventive Measures

AFB1 Toxicity in Human Food and Animal Feed Consumption: A Review of Experimental Treatments and Preventive Measures

Inland recreational fisheries contribute nutritional benefits and economic value but are vulnerable to climate change.

Inland recreational fishing is primarily considered a leisure-driven activity in freshwaters, yet its harvest can contribute to food systems. Here we estimate that the harvest from inland recreational fishing equates to just over one-tenth of all reported inland fisheries catch globally. The estimated total consumptive use value of inland recreational fish destined for human consumption may reach US$9.95 billion annually. We identify Austria, Canada, Germany and Slovakia as countries above the third quantile for nutrition, economic value and climate vulnerability. These results have important implications for populations dependent on inland recreational fishing for food. Our findings can inform climate adaptation planning for inland recreational fisheries, particularly those not currently managed as food fisheries.

Effects of position of seed perforation by cowpea weevils, Callosobruchus maculatus (F.), on viability and vigour of cowpea (Vigna unguiculata L. Walp) seed

Cowpea, Vigna unguiculata, is a high-protein legume crop that is often susceptible to pest damage. The cowpea weevil, Callosobruchus maculatus, predates on cowpea seeds in storage which renders grain unsuitable for human consumption. In the larval stage, cowpea weevils consume food reserves from the seed endosperm and may deter seed germination. The problem is more pervasive among resource-poor farmers in Sub-Saharan Africa, who grow cowpea for subsistence and keep the surplus grain for sale and use it as seed in the subsequent growing seasons. Unsuitable storage structures and ineffective methods of weevil control may increase weevil infestation of cowpea seeds in storage which constrains the production of cowpeas. The aim of the study was to determine if the position of seed perforation by cowpea weevils affects seed viability and growth of cowpea seedlings. Undamaged cowpea seeds ‘black-eyed peas’ were infested with cowpea weevils and stored for three months to allow for infestation. Thereafter, seeds with a single perforation or hole were selected from the infested seed lot and sorted into groups of damage according to the position of the hole on the seed. Five categories of potential damage were identified namely seeds with holes on the top, bottom, side, back or hilum area of the seed as presented schematically in the paper. The positions of holes on seeds served as treatments for the study and undamaged seed served as control. Data on germination and seedling height were collected and analysed statistically. Results reveal that the positions of damage on seed affects seed germination and seedling height. Seed germination on day two was significantly poorer for seeds damaged at the top, relative to both undamaged seeds and the alternative sites of damage. The results of top damaged seed being inferior than other treatments, remained consistent for germination on day seven and for seedling height at two weeks after the first emergence. Small holder farmers should therefore refrain from planting top damaged cowpea seed.

Nutritional, chemical, and morphological assessment of Couroupita guianensis: a potential edible fruit for human consumption

Nutritional, chemical, and morphological assessment of Couroupita guianensis: a potential edible fruit for human consumption

QTL Mapping and Candidate Gene Mining for Stem Diameter Using Genetic Basis of Cultivated Soybean and Wild Soybean

Soybean (Glycine max) is a vital food crop, serving as a major source of high-quality protein for human and animal consumption. Stem diameter is one of the primary determinants of the stem lodging resistance of a given plant, but there has been relatively little research to date focused on genes associated with this trait. To address this gap in the literature, 207 chromosome segment substitution lines (CSSLs) were generated in the present study through the crossing and backcrossing of the improved Suinong14 and the wild ZYD00006 soybean varieties. These CSSLs were then used for the mapping of quantitative trait loci (QTLs) associated with stem diameter in two-year field planting materials, leading to the identification of nine QTLs. Whole genome resequencing, RNA-seq, and qPCR were then used to evaluate candidate genes associated with stem diameter within these QTL intervals, ultimately leading to the selection of Glyma.04G004100 as a stem diameter-related gene. Subsequent qPCR analyses revealed that Glyma.04g004100 was upregulated in soybean plants with larger stem diameters, and haplotype analyses yielded results consistent with these stem diameter data in the population used to conduct this study. In summary, a series of QTLs associated with stem diameter were identified in the present study, resulting in the establishment of Glyma.04g004100 as a stem diameter-related gene. Together, these results offer a theoretical foundation for the future molecular-assisted breeding of lodging-resistant soybean varieties, and future functional research focused on Glyma.04g004100 may elucidate the molecular mechanisms and key signaling networks involved in soybean stem development.

In vitro genotoxicological evaluation of protein-rich powder derived from Xanthobacter sp. SoF1.

One way of limiting the environmental impact of food production and improving food security is to replace part of the animal- or plant-based protein in the human diet with protein sourced from microorganisms. The recently discovered bacterium Xanthobacter sp. SoF1 (VTT-E-193585) grows autotrophically using carbon dioxide gas as the only carbon source, yielding protein-rich biomass that can be processed further into a powder and incorporated into various food products. Since the safety of this microbial protein powder for human consumption had not been previously assessed, its genotoxic potential was evaluated employing three internationally recognized and standardized studies: a bacterial reverse mutation test, an in vitro chromosomal aberration assay in human lymphocytes, and an in vitro micronucleus test in human lymphocytes. No biologically relevant evidence of genotoxicity or mutagenicity was found.

Feed Friend: Fostering Community Involvement in Food Assistance

According to the Food and Agriculture Organization (FAO) of the United Nations, approximately one-third of all food produced for human consumption worldwide is wasted annually. Food wastage occurs at various stages of the supply chain, including production, processing, distribution, and consumption. FeedFriend is an innovative website which was designed to combat food-wastage by fostering a vibrant community of donors and recipients. Donor Registration Individuals or Businesses can sign up as Donors on FeedFriend, providing details about the surplus food they have available for donation. This includes restaurants with excess inventory, grocery stores with perishable items nearing expiration, or individuals with leftover food from events or meals. Food Listings Donors can create listings within the website, specifying the type and quantity of food they wish to donate, include relevant details such as expiration dates etc. In Feed Friend, Users can filter the Donation requests based on their location. This type of service is used to match donors with nearby recipients in need. Recipients may include food banks, shelters, community centers, or individuals facing food insecurity. Feed Friend facilitates communication between donors and recipients, coordinates pickup or delivery logistics directly by providing donor’s contact details. So that it enables a better communication between Donors and Receivers.

Passiflora nitida Kunth fruit: Chemical analysis, antioxidant capacity, and cytotoxicity

Passiflora nitida is a fruit-bearing species native to the Amazon region, considered an unconventional food plant, with its fruit commonly known as wild passion fruit. The aim of this study was to investigate the antioxidant properties, chemical profile, phenolic composition, and cytotoxicity of pulps and teas based on the pulp of P. nitida, contributing to the exploration of this species. This study provides important information about the chemical composition, which will contribute to the development of products based on wild passion fruit. The production of nonalcoholic beverages from the pulp with seeds is a viable alternative for utilizing the fruits with greater results, potentially offering a product with higher bioactive compounds and antioxidant capacity compared to seedless pulp. Moreover, P. nitida seed tea may constitute a significant source of nutrients for human consumption.

Enzyme-treated microalgal co-product diets for rainbow trout aquaculture: Supporting fish growth, phosphorus digestibility, and reducing phosphorus waste emission

Aquaculture is one of the fastest-growing food sectors, producing almost 50% of fish for human consumption worldwide. However, relying on fish meal and oil for aquaculture diets is not sustainable economically or environmentally. Aquaculture feeds also contain terrestrial plant ingredients with indigestible forms of phosphorus (P), of which 70%–80% can be released into aquatic environments. This P influx contributes to eutrophication of freshwater ecosystems that can lead to anoxic conditions. This study explores a more sustainable diet for salmonids, an important and valuable seafood. Our aim was to test ingredients with highly digestible forms of P in nutritionally balanced portions to support fish growth and reduce P loading. We determined the digestibility of three feeds containing raw, extruded, and enzymatically processed microalgal co-product of Nannochloropsis oculata compared to a conventional diet. We also quantified how much P was retained and excreted. We detected highest growth in trout fed enzymatically processed co-product feed, though it was not statistically different (p = 0.846) from growth of fish fed the reference or other co-product diets. The enzyme-treated, microalgal co-product ingredient and diet had comparable values for P digestibility and solid P excretion to the reference diet, but the lowest average solid P excretion of all test diets. Trout fed the enzyme-treated diet had the highest P retention, while the reference diet had the lowest (p = 0.0429). Trout fed the enzyme-treated diet had the lowest (p = 0.0174) and negative dissolved P excretion, while those fed the reference diet had the highest. Results showed that enzyme-treated N. oculata co-product maintains digestibility, increases P retention, and reduces dissolved P excretion compared to the reference diet in rainbow trout. These findings encourage follow-up research to design and test growth performance of diets containing enzyme-treated microalgal co-product as sustainable trout aquafeed.

Factors influencing subseasonal predictability of northern Eurasian cold spells

AbstractCold‐air outbreaks have significant impacts on human health, energy consumption, agriculture, and overall well‐being. This study aims to evaluate the effectiveness of Subseasonal‐to‐Seasonal (S2S) models in predicting cold conditions over northern Eurasia, defined here as the lower tercile of weekly mean 2‐metre temperature anomalies. To assess the predictability of these events we use ensemble hindcasts from five prediction systems from the S2S database. Our analysis focuses on identifying the conditions under which the models confidently predict cold temperatures with a high (>0.5) probability 3–4 weeks ahead, which potentially can represent windows of forecast opportunity. We compare the group of forecasts that correctly predicted the events to the group that forecasted events that did not occur in practice (false alarms). Most of the confident forecasts of cold spells, both correct and false alarms, have cold anomalies already in the initial conditions, often in conjunction with either a negative phase of the North Atlantic Oscillation, or Scandinavian Blocking. We find that S2S models tend to overpredict cold temperatures, with false alarms occurring more likely when the forecasts are initialized during a weak polar vortex. Furthermore, most of the confident false alarms receive the signal from the stratosphere rather than following internal tropospheric dynamics. False alarms initialized during the weak polar vortex conditions are more common when the vortex is in a recovery stage and, subsequently, the downward ‐propagating signal is short‐lived in the troposphere. The analysis of forecasts during different Madden–Julian Oscillation (MJO) phases shows that nearly half of all confident correct cold‐temperature forecasts are initialized during an active MJO in phases 6–8. On the other hand, most false alarms occur during phase 3, which we suggest is due to the presence of the Scandinavian Blocking regime in the initial conditions for this phase.

Assessment of water quality and associated human health risk of a tropical freshwater body in Edo State, Nigeria

The quality of freshwater sources is a key factor in sustainable development. This study investigated the water quality suitability of Ossiomo River for human consumption and the implications for public health. Water samples were collected and analysed from October 2021 to March 2022. Results indicated that the water physico-chemical parameters from sampled locations were within their respective National Environmental Standards and Regulation Enforcement Agency (NESRA) permissible limits except for pH (< 6.5), dissolved oxygen (< 6.0mg L−1), cadmium (> 0.005m /L−1), manganese (> 0.05mg L−1) and total hydrocarbon content (>10mg L−1). Comprehensive pollution index (0.4–1.0) and water quality index (251.57–765.37) values indicate that the water body was slightly polluted and unsuitable for human consumption (WQI > 50). Health risk assessment showed that children and adults were predisposed to non-carcinogenic risk (HIoral > 1) and carcinogenic risk (ICR: 1.21E-02 and 2.84E-03) from heavy metals through oral exposure. Natural run-off and anthropogenic activities were identified sources of heavy metals and hydrocarbons within the catchment. The study has provided data for water resource quality monitoring and raised concerns as to the need for immediate action to safeguard the public health of communities surrounding the Ossiomo River, through the provision of alternative sources of potable water.

The nutritional benefits of maize-soybean rotational systems in the North-Western Free State, South Africa

BackgroundMalnutrition is one of the major health concerns, particularly in the developing and under-developed world. In South Africa, maize is produced as a staple food and is the primary food for most of the country’s population. The North-Western Free State which forms part of the Nala municipality in the Lejweleputswa district of South Africa is a main producer of the country’s maize. However, the area is known for its sandy soil which contains little organic material, silt and clay. Maize in this area is normally grown in monoculture but with a focus on sustainable agriculture has recently incorporated soybean. As a means of fighting malnutrition, the objective of this study was to determine the influence that soybean incorporation as a rotation crop has on the nutritional value of maize.MethodsA trial was conducted on the farm Christinasrus in the North-Western Free State to compare the nutritional value of monoculture maize and maize in rotation with soybean over three consecutive seasons. Maize kernel samples were taken each season and its nutritional properties analyzed. Subsequent data were further analyzed using statistical analysis.ResultsResults showed that there was a seasonal effect on all nutritional properties with a general decrease in nutritional values in wetter years. Cropping systems had an effect on fibre content, with increased values observed in maize after soybean. In addition, there was a significant interaction between season and cropping systems on the total digestible nutritional value, with maize after soybean being more nutritious in wetter seasons.ConclusionResults suggest that maize in cropping systems with soybean has potential to be more nutritious while the soybean in the cropping system can act as a protein-rich companion, providing a more balanced diet for human consumption, thereby fighting malnutrition.

Mycobacterium avium subsp. paratuberculosis in Wild Boar (Sus scrofa) in Portugal

Paratuberculosis, or Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The agent was also found in wild mammals such as wild boar (Sus scrofa); however, the role of wild mammals in the epidemiology of MAP is unclear. During the research period, 941 free-ranging wild boar (S. scrofa) legally hunted in two locations in the central–eastern region of Portugal were examined. Ninety-seven wild boars exhibited one or more gross lesions and were tested for the presence of Mycobacterium avium subsp. paratuberculosis using acid-fast staining, mycobacterial culture, polymerase chain reaction (PCR), and histopathological examination. Forty-five animals (46.4%, 95% CI: 36.5–56.3%) were identified as infected, as indicated by positive results in culture and/or PCR. The findings revealed that the most significant risk factor was being a juvenile compared to yearlings and adults (OR = 10.2, 95% CI: 2.2–48.0). Based on our results, 37.9% (n = 11) of the infected animals were considered suitable for human consumption. Our findings offer novel insights into mycobacterial infections in wild boar populations in Portugal and suggest that wild boar could be a source of human infection if zoonotic potential is considered.

Lemna minor: Unlocking the Value of This Duckweed for the Food and Feed Industry

Duckweed (Lemna minor L.) is a small floating aquatic plant that has an important economic impact in several industrial areas. With its high biomass production, reasonable protein content, and resilience to several climates, it has been attracting increasing interest for potential use in animal and human food systems. Historically consumed in southwest Asia, this duckweed is now gaining attention as a potential novel food in Europe. This manuscript explores the contributions of duckweed to various food and feed industries, including aquaculture and livestock, while also pointing out the incipient research carried out for human consumption. Most importantly, it highlights the potential of Lemna minor as a vegetable for future human consumption whether eaten whole or through extraction of its nutrients.

Agro-industrial waste management employing benefits of artificial intelligence.

By 2050, the world's population is predicted to reach over 9 billion, which requires 70% increased production in agriculture and food industries to meet demand. This presents a significant challenge for the agri-food sector in all aspects. Agro-industrial wastes are rich in bioactive substances and other medicinal properties. They can be used as a different source for manufacturing products like biogas, biofuels, mushrooms, and tempeh, the primary ingredients in various studies and businesses. Increased importance is placed on resource recovery, recycling, and reusing (RRR) any waste using advanced technology like IoT and artificial intelligence. AI algorithms offer alternate, creative methods for managing agro-industrial waste management (AIWM). There are contradictions and a need to understand how AI technologies work regarding their application to AIWM. This research studies the application of AI-based technology for the various areas of AIWM. The current work aims to discover AI-based models for forecasting the generation and recycling of AIWM waste. Research shows that agro-industrial waste generation has increased worldwide. Infrastructure needs to be upgraded and improved by adapting AI technology to maintain a balance between socioeconomic structures. The study focused on AI's social and economic impacts and the benefits, challenges, and future work in AIWM. The present research will increase recycling and reproduction with a balance of cost, efficiency, and human resources consumption in agro-industrial waste management.

Nutritional Quality and Socio-Ecological Benefits of Mare Milk Produced under Grazing Management.

This review discusses the scientific evidence that supports the nutritional value of mare milk and how its properties are essentially achieved when mares are managed under grazing conditions. Mare milk's similarity with the chemical composition of human milk makes this food and its derived products not only suitable for human consumption but also an interesting food regarding human health. The contribution of horse breeding under grazing management to other socio-ecological benefits generated by equine farms is also highlighted. Both the high added value of mare milk and the socio-ecological benefits derived from pasture-based systems could be explored to improve the performance of equine farms located in arid and semi-arid areas or in regions with moderately harsh environmental conditions as equids have a strong adaptation capacity.

Potabilización de agua de lluvia para consumo humano: mitos y realidades

Water security is of paramount importance for social, economic, and environmental develop-ment in any region of the world. Rainwater har-vesting offers invaluable benefits to society mainly in rural areas which lack centralized water systems. At the same time, it is considered one of the solutions to solve the problem of water supply. The purification of rainwater does not represent great costs and many advantages. This article focuses on a literature review on the different myths about rainwater and its human consumption. The myths that are held about its qua-lity and provide information to motivate the collection of rainwater in homes considering the optimal conditions for its collection and storage.

Experimental Behavior of Solar Still Using Mixed Oxides Mn-Fe/Silicone Resin Composite as Selective Solar Absorber

In this research, the optical properties of a black pigment of mixed Mn-Fe oxides synthesized by the precipitation method dispersed in silicone resin at different concentrations for use as a solar absorber in a two-slope passive solar still were evaluated. The transmittance of different thicknesses of float glass with 0.1% Fe2O3 was also studied for use as a condenser in the construction of the solar still. Regarding the pigments, the absorbance and spectral emittance were evaluated, the floated. With the above information, both the absorbent surface and the condensers were optimized to propose the best design of the solar still. The novel hybrid material used as a selective coating in the still, which corresponds to 2.3% Mn-Fe mixed oxides, exhibited a high solar absorbance of 91.82% and a relatively low infrared thermal emission of less than 57.22%, as well as good stability to the corrosive and hot environment of the solar still, in addition to great durability over time and in real operating conditions. The float glass with the highest transmittance corresponds to a thickness of 3 mm and an average transmittance of 82.57%. The built prototype was characterized by obtaining the temperature profiles of the absorber and condensers, thermal efficiency, production and water quality. The prototype has a thermal efficiency of 29.6%, requiring only energy from the sun for its operation. The water produced by this device becomes an option for human use and consumption in accordance with current official standards, allowing supply in communities that do not have access to drinking water but have access to seawater or waters with high hardness.

Endocrine disruptors effects in ruminant reproduction

Oral exposure to synthetic and natural endocrine disruptors (ED) at different stages of life is related to alterations in the reproductive system. Ruminants represent a model for studying the effects of ED in humans due to some similarities in their development. Ruminants are considered of great importance due to continuous exposure to pasture and contaminated soils and human consumption of their meat. The present review aims to describe ED effects in ruminants’ reproduction, when they are consumed in the perinatal, postnatal-pre-adulthood, and adult stages. Feeding with phytoestrogens (PE) rich forage has no nocive consequences on the male reproductive system at any stages of life; in females, they produce more significant harmful effects when administered in adulthood. In the perinatal and postnatal-pre-adulthood stages, negative effects of synthetic ED were found in both males and females. This review presents study opportunities for further research related to oral exposure to ED.